Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars

(1) Background: Although resistance to pathogens and pests has been researched in many potato cultivars breeding lines with DNA markers, there is scarce evidence as the efficiency of marker-assisted selection (MAS) for these traits when applied at early stages breeding. A goal this study was estimate potential affordable markers track genes that are effective against pathogen Phytophthora infestans (Rpi genes), a practical tool on progeny 68 clones derived from cross between cultivar Sudarynya hybrid 13/11-09. (2) Methods: population studied four years elucidate distribution late blight (LB) other agronomical desirable or simple phenotype such tuber flower pigmentation, yield capacity structure. LB phenotypically evaluated following natural artificial infection presence/absence nine Rpi assessed 11 sequence-characterized amplified region (SCAR) markers. To validate analysis, profile 13/11-09 parent established using diagnostic gene enrichment sequencing (dRenSeq) gold standard. (3) Results: program, screening segregation F1 offspring, MAS can halve workload selected SCAR provide useful tools.